GINS4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GINS4 Antibody [NBP2-16659] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: GINS4 Antibody [NBP2-16659] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.

GINS4 Antibody [NBP2-16659] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SLD5 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GINS4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GINS4 Antibody - BSA Free (NBP2-16659) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GINS4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GINS4. The exact sequence is proprietary.
• Localization: Cytoplasm (By similarity); Nucleus
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GINS4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%), Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GINS4 Antibody - BSA Free

• DNA replication complex GINS protein SLD5
• GINS complex subunit 4 (Sld5 homolog)
• GINS complex subunit 4
• SLD5 homolog
• SLD5MGC14799

Background

The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GINS4 Antibody (NBP2-16659)(2)

Publications using NBP2-16659

• Chatterjee N, Fraile-Bethencourt E, Baris A et al. MicroRNA-494 Regulates Endoplasmic Reticulum Stress in Endothelial Cells Frontiers in Cell and Developmental Biology 2021-07-12 [PMID: 34322482]
• Anand S, Chatterjee N, Espinosa-Diez C A miR-494 dependent feedback loop regulates ER stress BioRxiv 2020-01-01 (ICC/IF, Human)

Bioinformatics

• Gene Symbol: GINS4
• Uniprot:
  • Q9BRT9(Human)