PRR5 Antibody - BSA Free

Immunoprecipitation:PRR5 AntibodyNBP3-28984] -Detection of human Protor-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Protor-1 antibody NBP3-28984 (lot NBP3-28984-1) used for IP at 6 µg/mg lysate. Protor-1 was also immunoprecipitated by rabbit anti-Protor-1 antibodies . For blotting immunoprecipitated Protor-1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRR5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PRR5 Antibody - BSA Free (NBP3-28984) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 225 and 275
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRR5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for PRR5 Antibody - BSA Free

• FLJ20185
• FLJ20185k
• PP610
• proline rich 5 (renal)
• protein observed with Rictor-1
• Protor-1
• PRR5

Background

This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRR5