PRR7 Antibody (TRAP3/10) - BSA Free

Images

 
Western Blot: PRR7 Antibody (TRAP3/10) [NBP1-30424] - Detection of PRR7 / TRAP3 in murine brain lysate by Western blotting using the monoclonal antibody TRAP3/10.
Immunocytochemistry/ Immunofluorescence: PRR7 Antibody (TRAP3/10) [NBP1-30424] - Staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
TRAP3/10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

PRR7 Antibody (TRAP3/10) - BSA Free Summary

Immunogen
Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)
Specificity
The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRR7
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Western Blot 1 ug/ml
Application Notes
Immunocytochemistry: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PRR7 Antibody (TRAP3/10) - BSA Free

  • MGC10772
  • proline rich 7 (synaptic)
  • proline-rich protein 7
  • Synaptic proline-rich membrane protein

Background

PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRR7