PRPF6 Antibody

Western Blot: PRPF6 Antibody [NBP1-46212] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PRP6 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody NBP1-46213, which recognizes a downstream epitope.

Immunohistochemistry: PRPF6 Antibody [NBP1-46212] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv, Pm-Or
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PRPF6 Antibody Summary

• Description: Novus Biologicals Rabbit PRPF6 Antibody (NBP1-46212) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human Pre-mRNA Processing Factor 6 using the numbering given in entry NP_036601.2 (GeneID 24148).
• Predicted Species: Rat (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:1,000
  • Immunohistochemistry-Paraffin 1:100 to 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRPF6 Antibody

• androgen receptor N-terminal domain transactivating protein-1
• ANT-1
• bB152O15.1
• C20orf14
• chromosome 20 open reading frame 14
• hPrp6
• p102 U5 small nuclear ribonucleoprotein particle-binding protein
• pre-mRNA-processing factor 6
• PRP6 homolog
• PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
• PRP6 pre-mRNA processing factor 6 homolog (yeast)
• Prp6
• putative mitochondrial outer membrane protein import receptor
• TOM
• U5 snRNP-associated 102 kDa protein
• U5-102 kDa protein
• U5-102K

Background

The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF6