PRPF4B Antibody

Images

 
Western Blot: PRPF4B Antibody [NB100-86997] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.04 ug/ml.
Immunohistochemistry: PRPF4B Antibody [NB100-86997] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PRPF4B used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: PRPF4B Antibody [NB100-86997] - FFPE section of human thyroid carcinoma. Affinity purified rabbit anti-PRPF4B used at a dilution of 1:250. Detection: DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
   

Biological Strategies

     

Order Details

PRPF4B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human PRP4 pre-mRNA processing factor 4 homolog B using the numbering given in entry NP_003904.3 (GeneID 8899).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRPF4B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
PRPF4B Lysate (NBL1-14823)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRPF4B Antibody

  • dJ1013A10.1 (PRP4 protein kinase homolog)
  • dJ1013A10.1
  • KIAA0536EC 2.7.11.1
  • PR4H
  • protein-serine/threonine kinase
  • PRP4 kinase
  • PRP4 pre-mRNA processing factor 4 homolog B (yeast)
  • PRP4 pre-mRNA-processing factor 4 homolog
  • PRP4H
  • PRP4K
  • PRP4Prp4
  • serine/threonine-protein kinase PRP4 homolog

Background

Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRPF4B
Entrez
Uniprot