PRPF4B Antibody

Western Blot: PRPF4B Antibody [NB100-86997] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.04 ug/ml.

Immunohistochemistry-Paraffin: PRPF4B Antibody [NB100-86997] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- PRPF4B used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Pm
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PRPF4B Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human PRP4 pre-mRNA processing factor 4 homolog B using the numbering given in entry NP_003904.3 (GeneID 8899).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPF4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 -1:1000
  • Immunohistochemistry-Paraffin 1:200 -1:1000
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.
• Control:

  PRPF4B Overexpression Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRPF4B Antibody

• dJ1013A10.1 (PRP4 protein kinase homolog)
• dJ1013A10.1
• KIAA0536EC 2.7.11.1
• PR4H
• protein-serine/threonine kinase
• PRP4 kinase
• PRP4 pre-mRNA processing factor 4 homolog B (yeast)
• PRP4 pre-mRNA-processing factor 4 homolog
• PRP4H
• PRP4K
• PRP4Prp4
• serine/threonine-protein kinase PRP4 homolog

Background

Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF4B
• Entrez:
  • 8899(Human)
• Uniprot:
  • Q13523(Human)