PRPF31 Antibody

Images

 
Western Blot: PRPF31 Antibody [NBP1-51909] - Analysis of PRPF31 in cell line A431 lysate using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PRPF31 Antibody [NBP1-51909] - (0.3ug/ml) staining of cell line A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

PRPF31 Antibody Summary

Immunogen
Peptide with sequence C-LKVKGEKSGLMST corresponding to C-Terminus according to NP_056444.3.
Epitope
C-LKVKGEKSGLMST
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRPF31
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 60 kDa band observed in A431 lysates (calculated MW of 55.4 kDa band according to NP_056444.3.
Publications
Read Publication using NBP1-51909.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PRPF31 Antibody

  • DKFZp566J153
  • hPrp31
  • NY-BR-99
  • Pre-mRNA-processing factor 31
  • Protein 61K
  • PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
  • PRP31 pre-mRNA processing factor 31 homolog (yeast)
  • PRP31RP11
  • Serologically defined breast cancer antigen NY-BR-99
  • U4/U6 small nuclear ribonucleoprotein Prp31
  • U4/U6 snRNP 61 kDa protein

Background

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 (MIM 180692) RNAs and the 15.5K protein (NHP2L1; MIM 601304). The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state (Liu et al., 2007) [PubMed 17412961].[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRPF31 Antibody (NBP1-51909)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRPF31
Entrez
Uniprot