Protocadherin gamma C3 Antibody (S174B-27)

Western Blot: Protocadherin gamma C3 Antibody (S174B-27) [NBP2-42203] - analysis of Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3 showing detection of 100 kDa Protocadherin Gamma C3 protein using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 100 kDa.

Immunocytochemistry/Immunofluorescence: Protocadherin gamma C3 Antibody (S174B-27) [NBP2-42203] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.

Product Discontinued

Protocadherin gamma C3 Antibody (S174B-27) Summary

• Immunogen: Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-C3. Human: 97% identity (83/85 amino acids identical). Rat: 97% identity (83/85 amino acids identical). No significant identity with other Gamma-protocadherin proteins.
• Localization: Cell Membrane
• Specificity: No significant identity with other Gamma-protocadherin proteins.Detects approx 100kDa.
• Predicted Species: Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCDHGC3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: 1 ug/ml of Protocadherin Gamma C3 Antibody was sufficient for detection of Protocadherin Gamma C3 in 20 ug of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma C3 plasmids) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Protocadherin gamma C3 Antibody (S174B-27)

• Cadherin-Like 2
• PC43
• PC-43
• PCDH2
• PCDH-GAMMA-C3
• PCDHGC3
• protocadherin 2
• Protocadherin 43
• Protocadherin gamma C3
• protocadherin gamma subfamily C, 3
• protocadherin gamma-C3
• Protocadherin-2
• protocadherin-43

Background

The PCDHGC3 gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked onchromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism maybe involved in their regulat

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDHGC3
• Uniprot:
  • Q91XX1(Human)