RhoB Antibody

Images

 
Western Blot: RhoB Antibody [NBP2-20154] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RhoB antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: RhoB Antibody [NBP2-20154] - detects RHOB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft.
Western Blot: RhoB Antibody [NBP2-20154] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:500.
Western Blot: RhoB Antibody [NBP2-20154] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Sh, XpSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RhoB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RhoB. The exact sequence is proprietary.
Localization
Late endosome membrane; Lipid-anchor; Cell membrane; Lipid-anchor; Nucleus
Predicted Species
Porcine (100%), Bovine (100%), Chicken (97%), Canine (99%), Sheep (100%), Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RHOB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (90%), Rhesus Monkey (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for RhoB Antibody

  • ARH6MSTP081
  • ARHBAplysia RAS-related homolog 6
  • h6
  • MST081
  • oncogene RHO H6
  • ras homolog gene family, member B
  • Rho cDNA clone 6
  • RHOH6RhoB
  • rho-related GTP-binding protein RhoB

Background

Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for RhoB Antibody (NBP2-20154)

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Blogs on RhoB.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

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Bioinformatics

Gene Symbol RHOB
Uniprot