Mouse Prothrombin ELISA Kit (Colorimetric)

ELISA: Mouse Prothrombin ELISA Kit (Colorimetric) [NBP2-60635] - Standard curve

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Urine
• Standard Curve Range: 0.781-50 ng/ml (example only; lot dependent)
• Sensitivity: 0.75 ng/ml (example only; lot dependent)

Mouse Prothrombin ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Specificity: Mouse Prothrombin
• Standard Curve Range: 0.781-50 ng/ml (example only; lot dependent)
• Sensitivity: 0.75 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.70% (example only; lot dependent)
• Intra-Assay: CV% < 4.00% (example only; lot dependent)
• Spike Recovery: 97.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: F2

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Prothrombin ELISA Kit (Colorimetric)

• coagulation factor II (thrombin)
• Coagulation factor II
• EC 3.4.21
• EC 3.4.21.5
• prothrombin B-chain
• prothrombin
• PT
• serine protease

Background

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: F2