Human Prothrombin ELISA Kit (Colorimetric)

ELISA: Human Prothrombin ELISA Kit (Colorimetric) [NBP2-60590] - Standard Curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Urine, Saliva, Milk, CSF
• Standard Curve Range: 0.344 - 22 ng/mL (example only; lot dependent)
• Sensitivity: 0.2 ng/ml (example only; lot dependent)

Human Prothrombin ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Specificity: Human prothrombin
• Standard Curve Range: 0.344 - 22 ng/mL (example only; lot dependent)
• Sensitivity: 0.2 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 8.80% (example only; lot dependent)
• Intra-Assay: CV% < 4.80% (example only; lot dependent)
• Spike Recovery: 100.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: F2

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Prothrombin ELISA Kit (Colorimetric)

• coagulation factor II (thrombin)
• Coagulation factor II
• EC 3.4.21
• EC 3.4.21.5
• prothrombin B-chain
• prothrombin
• PT
• serine protease

Background

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Prothrombin ELISA Kit (NBP2-60590)(2)

Publications using NBP2-60590

• Beernink RHJ, Schuitemaker JHN, Zwertbroek EF et al. Early pregnancy biomarker discovery study for spontaneous preterm birth Placenta 2023-06-19 [PMID: 37356366]
• Krenzlin H, Frenz C, Schmitt J et al. High CSF thrombin concentration and activity is associated with an unfavorable outcome in patients with intracerebral hemorrhage PLoS One 2020-11-11 [PMID: 33175864] (ELISA, Human)
  
  Details:
  ELISA performed on cerebrospinal fluid from human patients who experienced intracerebral hemorrhage.

FAQs for Prothrombin ELISA Kit (NBP2-60590). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: F2