CD177 Antibody (4C4) - Azide and BSA Free

Western Blot: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody (M01), clone 4C4.Lane 1: CD177 transfected lysate(46.4 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01] - Analysis of monoclonal antibody to CD177 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.

Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD177 MaxPab rabbit polyclonal antibody.

ELISA: CD177 Antibody (4C4) [H00057126-M01] - Detection limit for recombinant GST tagged CD177 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 4C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CD177 Antibody (4C4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CD177 Antibody (4C4) - Azide and BSA Free (H00057126-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and IP. Anti-CD177 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CD177 (AAH29167, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ALLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESGPQVSLVLSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLW
• Specificity: CD177 (4C4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD177
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CD177 Antibody (4C4) - Azide and BSA Free

• CD177 antigenNB1 GP
• CD177 molecule
• CD177
• HNA-2a
• HNA2Acell surface receptor
• Human neutrophil alloantigen 2a
• NB1 GP
• NB1PRV-1
• polycythemia rubra vera 1
• Polycythemia rubra vera protein 1
• PRV1
• PRV1NB1 glycoprotein

Background

NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD177 Antibody (H00057126-M01)(2)

Publications using H00057126-M01

• Silvestre-Roig C, Braster Q, Wichapong K et al. Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death. Nature. 2019-05-01 [PMID: 31043745]
• Toyoda T, Tsukamoto T, Yamamoto M et al. Gene expression analysis of a Helicobacter pylori-infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer. BMC Gastroenterol. 2013-07-30 [PMID: 23899160]

Bioinformatics

• Gene Symbol: CD177
• Entrez:
  • 57126(Human)
• OMIM:
  • 162860(Human)
• Uniprot:
  • AAH29167()