Proteinase 3/Myeloblastin/PRTN3 Antibody (684042)

Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 1 µg/mL of Human Proteinase 3/Myeloblastin/PRTN3 Mono-clonal Antibody (Catalog # MAB6134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 684042
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Proteinase 3/Myeloblastin/PRTN3 Antibody (684042) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf21-derived recombinant human Proteinase 3/Myeloblastin/PRTN3
  Ala26-Arg249
  Accession # P24158
• Specificity: Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Granzyme B is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRTN3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proteinase 3/Myeloblastin/PRTN3 Antibody (684042)

• ACPA
• AGP7
• AGP7PR3
• azurophil granule protein 7
• C-ANCA
• EC 3.4.21
• EC 3.4.21.76
• Leukocyte proteinase 3
• MBN
• MBT
• Myeloblastin
• Neutrophil proteinase 4
• NP4
• NP-4
• P29C-ANCA antigen
• PR-3CANCA
• Proteinase 3
• PRTN3
• serine proteinase, neutrophil
• Wegener autoantigen
• Wegener granulomatosis autoantigen

Background

Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C‑terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proteinase 3/Myeloblastin/PRTN3 Antibody (MAB6134)(1)

Publication using MAB6134

• YJ Choi, S Kim, Y Choi, TB Nielsen, J Yan, A Lu, J Ruan, HR Lee, H Wu, B Spellberg, JU Jung SERPINB1-mediated checkpoint of inflammatory caspase activation Nat. Immunol., 2019-01-28;0(0):. 2019-01-28 [PMID: 30692621] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PRTN3
• Uniprot:
  • P24158(Human)