Proteinase 3/Myeloblastin/PRTN3 Antibody (684042) [Alexa Fluor® 488]

• Applications: WB
• Clone: 684042
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Proteinase 3/Myeloblastin/PRTN3 Antibody (684042) [Alexa Fluor® 488] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf21-derived recombinant human Proteinase 3/Myeloblastin/PRTN3
  Ala26-Arg249
  Accession # P24158
• Specificity: Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Granzyme B is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C‑terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.