Proteasome 20S alpha 6 Antibody (OTI3B8)

Images

 
Western Blot: Proteasome 20S alpha 6 Antibody (3B8) [NBP2-45419] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6.
Immunohistochemistry: Proteasome 20S alpha 6 Antibody (3B8) [NBP2-45419] - Analysis of Human Kidney tissue.
Immunohistochemistry: Proteasome 20S alpha 6 Antibody (3B8) [NBP2-45419] - Analysis of Carcinoma of Human kidney tissue.
Immunohistochemistry: Proteasome 20S alpha 6 Antibody (3B8) [NBP2-45419] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: Proteasome 20S alpha 6 Antibody (3B8) [NBP2-45419] - Analysis of Human pancreas tissue.
Immunohistochemistry: Proteasome 20S alpha 6 Antibody (3B8) [NBP2-45419] - Analysis of Carcinoma of Human prostate tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Proteasome 20S alpha 6 Antibody (OTI3B8) Summary

Immunogen
Full length human recombinant protein of human PSMA6 (NP_002782) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSMA6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:500
Theoretical MW
27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Proteasome 20S alpha 6 Antibody (OTI3B8)

  • EC 3.4.25.1
  • IOTA
  • Macropain iota chain
  • macropain subunit iota
  • MGC22756
  • MGC2333
  • MGC23846
  • Multicatalytic endopeptidase complex iota chain
  • p27K
  • p27KPROS-27
  • PROS27
  • PROS-27
  • PROS2727 kDa prosomal protein
  • prosomal P27K protein
  • proteasome (prosome, macropain) subunit, alpha type, 6
  • Proteasome 20S alpha 6
  • Proteasome iota chain
  • proteasome subunit alpha type-6
  • proteasome subunit iota
  • PSMA6

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMA6