Proteasome 20S alpha 5 Antibody

Images

 
Western Blot: Proteasome 20S alpha 5 Antibody [NB120-11437] - Analysis of HeLa cell extract. Lane 1 is NB120-11437. Lane 2 is NB120-11437 pre-incubated with peptide.
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 5 Antibody [NB120-11437] - HeLa cells

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Proteasome 20S alpha 5 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5.
Localization
Cytoplasmic and Nuclear
Specificity
This detects proteasome 20S subunit alpha 5 from human, rat and mouse cell lysates.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
WB: Detects an approx. 26.5 kDa protein.
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Expected cross-reactivity with Zebrafish (100%) base on sequence homology.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 1mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Proteasome 20S alpha 5 Antibody

  • EC 3.4.25.1
  • FLJ42315
  • macropain subunit zeta
  • Macropain zeta chain
  • MGC117302
  • MGC125802
  • MGC125803
  • MGC125804
  • Multicatalytic endopeptidase complex zeta chain
  • proteasome (prosome, macropain) subunit, alpha type, 5
  • proteasome alpha 5 subunit
  • proteasome component 5
  • proteasome subunit alpha type-5
  • proteasome subunit zeta
  • Proteasome zeta chain
  • PSC5
  • ZETA

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMA5
Entrez