Proteasome 19S S3 Antibody

Images

 
Western Blot: Proteasome 19S S3 Antibody [NBP1-31530] - NIH-3T3, with 7.5% SDS PAGE, diluted at 1:1000.
Immunohistochemistry-Paraffin: Proteasome 19S S3 Antibody [NBP1-31530] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: Proteasome 19S S3 Antibody [NBP1-31530] - Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE, antibody diluted at 1:500.
Western Blot: Proteasome 19S S3 Antibody [NBP1-31530] - 293T (lane A), A431 (lane B), and H1299 (lane C), with 7.5% SDS PAGE, diluted at 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.23 mg/ml

Order Details

Proteasome 19S S3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.23 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Proteasome 19S S3 Antibody

  • 26S proteasome regulatory subunit RPN3
  • 26S proteasome regulatory subunit S3
  • P58
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
  • Proteasome subunit p58
  • Rpn3
  • S3, 26S proteasome non-ATPase regulatory subunit 3
  • tissue specific transplantation antigen 2
  • TSTA2

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMD3