Recombinant Mouse Prostatic Acid Phosphatase/ACPP His (C-Term) Protein

SDS-Page: Recombinant Mouse Prostatic Acid Phosphatase/ACPP His (C-Term) Protein [NBP3-05692] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Discontinued

Recombinant Mouse Prostatic Acid Phosphatase/ACPP His (C-Term) Protein Summary

• Description: A recombinant protein His-tagged at the C-terminus corresponding to 32-381 amino acids of Prostatic Acid Phosphatase/ACPP
  
  

  Source:Baculovirus

  Amino acid sequence: KELKFVTLVF RHGDRGPIET FPTDPITESS WPQGFGQLTQ WGMEQHYELG SYIRKRYGRF LNDTYKHDQI YIRSTDVDRT LMSAMTNLAA LFPPEGISIW NPRLLWQPIP VHTVSLSEDR LLYLPFRDCP RFEELKSETL ESEEFLKRLH PYKSFLDTLS SLSGFDDQDL FGIWSKVYDP LFCESVHNFT LPSWATEDAM IKLKELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILKNMK LATQPQKYKK LVMYSAHDTT VSGLQMALDV YNGVLPPYAS CHMMELYHDK GGHFVEMYYR NETQNEPYPL TLPGCTHSCP LEKFAELLDP VISQDWATEC MATSSHQGRN

• Details of Functionality: Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: ACP3
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Less than 1 EU/ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 41.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse Prostatic Acid Phosphatase/ACPP His (C-Term) Protein

• acid phosphatase, prostate
• ACP3
• ACP-3
• ACPP
• EC 3.1.3.2
• PAP
• Prostatic Acid Phosphatase
• prostatic acid phosphotase

Background

Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase. This antibody does not inhibit the enzymatic activity of PSAP. It reacts with non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no cross reaction with other phosphatases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ACP3