Prohibitin Antibody (SR46-02)

Western Blot: Prohibitin Antibody (SR46-02) [NBP2-67334] - Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room tem

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (SR46-02) [NBP2-67334] - PDD005 enhances prohibitin expression in aged mice. Images showing prohibitin (red) expression in SGZ; blue = nuclei. 40X magnification, scale bar = 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-57560-3), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Prohibitin Antibody (SR46-02) [NBP2-67334] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-31, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Prohibitin Antibody (SR46-02) [NBP2-67334] - Analysis of HepG2 cells with Prohibitin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seco antibody

Western Blot: Prohibitin Antibody (SR46-02) [NBP2-67334] - Analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (SR46-02) [NBP2-67334] - Staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (SR46-02) [NBP2-67334] - Staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Prohibitin Antibody (SR46-02) [NBP2-67334] - Analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Prohibitin Antibody (SR46-02) [NBP2-67334] - Analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Prohibitin Antibody (SR46-02) [NBP2-67334] - Analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Prohibitin Antibody (SR46-02) [NBP2-67334] - Analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Prohibitin Antibody (SR46-02) [NBP2-67334] - Analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Prohibitin Antibody (SR46-02) [NBP2-67334] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-31, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Prohibitin Antibody (SR46-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Prohibitin aa 147-196 / 272. (SwissProt: P35232 Human; SwissProt: P67778 Mouse; SwissProt: P67779 Rat; SwissProt: Q7T1D8 Zebrafish)
• Localization: Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PHB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31980673). IP: Use at an assay dependent concentration.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Prohibitin Antibody (SR46-02)

• PHB
• PHB1
• Prohibitin

Background

Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prohibitin Antibody (NBP2-67334)(1)

Publication using NBP2-67334

• Guyot AC, Leuxe C, Disdier C et al. A Small Compound Targeting Prohibitin with Potential Interest for Cognitive Deficit Rescue in Aging mice and Tau Pathology Treatment Sci Rep 2020-01-24 [PMID: 31980673] (IHC-Fr)

Bioinformatics

• Gene Symbol: PHB1