Prohibitin Antibody (II-14-10)

Western Blot: Prohibitin Antibody (II-14-10) [NB600-1292] - Metformin activates apoptotic cell death; upper panel: MDA-MB-231 cells were kept in the presence or absence of metformin for the time indicated and then processed to obtain mitochondrial fractions. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as loading and purity control, respectively. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/1/49) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Prohibitin Antibody (II-14-10) [NB600-1292] - Formalin fixed paraffin embedded liver hepato cellular carcinoma stained with Prohibitin antibody (NB600-1292).

Product Discontinued

Prohibitin Antibody (II-14-10) Summary

• Immunogen: BALB/C mice were injected with purified recombinant rat prohibitin protein.
• Localization: Cytoplasmic
• Marker: Mitochondria Marker
• Specificity: This is specific to 30kD protein known as Prohibitin. Prohibitin is an evolutionarily conserved protein located in the innermembrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression. This antibody cross reacts with mouse, rat, and pig.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot
• Application Notes: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method.
  We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in Western blot reported in scientific literature (PMID 22595756).

Reactivity Notes

This antibody is reactive against pig. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Prohibitin Antibody (II-14-10)

• PHB
• PHB1
• Prohibitin

Background

Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prohibitin Antibody (NB600-1292)(4)

Publications using NB600-1292

• Saladini S, Aventaggiato M, Barreca F et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells 2019-01-14 [PMID: 30646605] (WB, Human)
• Lugini L, Cecchetti S, Huber V et al. Immune surveillance properties of human NK cell-derived exosomes. J Immunol. [PMID: 22904309] (WB, Human)
  
  Details:
  Citation using the IHC-Prediluted form of this antibody.
• Pellegrini L, Pucci B, Villanova L et al. SIRT3 protects from hypoxia and staurosporine-mediated cell death by maintaining mitochondrial membrane potential and intracellular pH Cell Death Differ [PMID: 22595756] (WB, Human)
  
  Details:
  Used the IHC-Prediluted form of this antibody.
• Xu Z, Wu J, Zha X. Up-regulation of prohibitin 1 is involved in the proliferation and migration of liver cancer cells. Sci China Life Sci. [PMID: 21318481] (WB, Human)
  
  Details:
  Citation using the IHC-Prediluted form of this antibody.

Bioinformatics

• Gene Symbol: PHB1
• Entrez:
  • 5245(Human)
  • 18673(Mouse)
  • 25344(Rat)