Progesterone R/NR3C3 Antibody (PR484)

Images

 
Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Formalin-paraffin normal human breast carcinoma stained with Progesterone Receptor Ab (PR484).
Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Simple Western lane view shows a specific band for Progesterone R/NR3C3 in 0.2 mg/ml of T47d (left) and MCF-7 (right) lysate(s). This experiment was ...read more
Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Electropherogram image of the corresponding Simple Western lane. Progesterone R/NR3C3 antibody was used at 10 ug/ml dilution of T47d and MCF-7 ...read more
SDS-Page: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC-P, PAGE
Clone
PR484
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Progesterone R/NR3C3 Antibody (PR484) Summary

Immunogen
A progesterone receptor recombinant protein was used as the immunogen for the antibody. The specific epitope has not been mapped.
Localization
Nucleus
Marker
Marker of Progestin Dependence
Specificity
This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PGR
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Simple Western 10 ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • SDS-Page
Application Notes
Human Chromosome Location: 11q22.1
Mol. Weight of Antigen: PR-A (81kDa) and PR-B (116kDa).

Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Progesterone R/NR3C3 Antibody (PR484)

  • NR3C3
  • NR3C3PRNuclear receptor subfamily 3 group C member 3
  • PGR
  • Progesterone R
  • progesterone receptor
  • ProgesteroneR

Background

The progesterone receptor (also known as NR3C3) is a member of the steroid or nuclear hormone receptor superfamily family of proteins. Family members are involved in diverse physiological functions including embryonic development, cell differentiation, and homeostasis. The receptors function by binding to DNA in the nucleus and regulating the transcription of target genes. The progesterone receptor is activated by its ligand progesterone, which is a steroid hormone involved in the menstrual cycle, pregnancy and embryogenesis. Progesterone receptor expression status is commonly used as a marker for classifying breast cancers and for predicting prognosis and response to endocrine therapy. That is, antibody positive versus antibody negative tumors can have potential implications with respect to predicted outcome, treatment, or disease monitoring. The antibody reacts with the progesterone receptor. Progesterone receptor is expressed as two major isoforms, PR-A (81 kDa) and PR-B (116 kDa). These isoforms are generated from alternative transcriptional start sites within the progesterone receptor gene. Both forms are ligand activated by progesterone although they differ in their relative ability to activate target gene transcription. Antibody to progesterone receptor is widely used to study breast and other progesterone receptor containing tissues such as the endometrium. Researchers are encouraged to consult the scientific literature for additional information regarding the use of antibody to measure progesterone receptor as part of assessing breast or other tumor types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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FAQs for Progesterone R/NR3C3 Antibody (NBP2-33324). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
    • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

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Bioinformatics

Gene Symbol PGR
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