Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

Western Blot: Progesterone Receptor [p Ser294] Antibody (608) [NB200-320] - Whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).

• Reactivity: Hu, Pm
• Applications: WB, IHC
• Clone: 608
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free

Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free Summary

• Description: Novus Biologicals Mouse Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free (NB200-320) is a monoclonal antibody validated for use in IHC and WB. Anti-Progesterone R/NR3C3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH. Accession # P06401
• Modification: p Ser294
• Localization: Nuclear
• Specificity: Specific for endogenous levels of the ~90 kDa Progesterone R/NR3C3-A isoform and the ~120 kDa Progesterone R/NR3C3-B isoform phosphorylated at Ser294. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000
  • Western Blot 1:1000
• Application Notes: Recognizes a ~115 kDa doublet/triplet and also a 80 kDa single band in western blots.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Progesterone R/NR3C3 [p Ser294] Antibody (608) - Azide Free

• NR3C3
• NR3C3PRNuclear receptor subfamily 3 group C member 3
• PGR
• Progesterone R
• progesterone receptor
• ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Progesterone R/NR3C3 Antibody (NB200-320)(2)

Publications using NB200-320

• Cho G, Lim Y, Golden JA. SUMO interaction motifs in Sizn1 are required for promyelocytic leukemia protein nuclear body localization and for transcriptional activation. J Biol Chem 2009-07-17 [PMID: 19416967] (Human)
• Perez M, Cuadros R, Benitez MJ, Jimenez JS. Interaction of Alzheimer's disease amyloid beta peptide fragment 25-35 with tau protein, and with a tau peptide containing the microtubule binding domain. J Alzheimers Dis 2004-10-01 [PMID: 15505366] (Human)

FAQs for Progesterone R/NR3C3 Antibody (NB200-320). (Showing 1 - 1 of 1 FAQs).

1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
   • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Bioinformatics

• Gene Symbol: PGR
• Entrez:
  • 5241(Human)
• Uniprot:
  • P06401()