Progesterone R B/NR3C3 Antibody (CL8721) - Azide and BSA Free

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Orthogonal Strategies: Analysis in human endometrium and pancreas tissues using NBP3-44136 antibody. Corresponding Progesterone R B/NR3C3 RNA-seq data are presented for the same tissues.
Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Staining of human cerebral cortex shows no positivity in neurons as expected.
Staining of human endometrium shows strong nuclear positivity in glandular cells.
Staining of human prostate shows moderate to strong nuclear positivity in smooth muscle cells.
Staining of human pancreas shows moderate to strong nuclear positivity in Langerhans cells.
Analysis in human cell line TD47D.
Staining of T47D cells using the Anti-Progesterone R B/NR3C3 monoclonal antibody) .

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL8721
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

 

Order Details

Progesterone R B/NR3C3 Antibody (CL8721) - Azide and BSA Free Summary

Immunogen
This antibody was generated using a recombinant protein sequence of P06401, with the exact immunogen sequence remaining proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PGR
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Progesterone R B/NR3C3 Antibody (CL8721) - Azide and BSA Free

  • PGRB
  • Progesterone R B
  • Progesterone RB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PGR