Human Profilin 1 ELISA Kit (Colorimetric)

ELISA: Human Profilin 1 ELISA Kit (Colorimetric) [NBP2-81243] - Samples were spiked with high concentrations of Human Profilin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Profilin 1 ELISA Kit (Colorimetric) [NBP2-81243] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Human Profilin 1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Profilin 1 in samples. No significant cross-reactivity or interference between Human Profilin 1 and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.12%
• Intra-Assay: CV% < 4.85%
• Spike Recovery: 87-108%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PFN1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Profilin 1 ELISA Kit (Colorimetric)

• PFN1
• PROF1
• Profilin 1
• Profilin I
• profilin-1

Background

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PFN1