ProDots Recombinant Human/Mouse/Rat Activin A Protein

ProDots® Recombinant Human/Mouse/Rat Activin A induces hemoglobin expression in K562 chronic myelogenous leukemia cells. The ED50 is 0.2-1.2 ng/mL.

1µg/lane of ProDots® Recombinant Human/Mouse/Rat Activin A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing single bands at 14 kDa and 24 kDa, respectively.

Product Discontinued

ProDots Recombinant Human/Mouse/Rat Activin A Protein Summary

• Additional Information: Supplied as ready-to-use 5 ug protein balls
• Details of Functionality: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED₅₀ for this effect is 0.2-1.2 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived Activin A protein
  Gly311-Ser426
• Accession #: P08476
• N-terminal Sequence: Gly311
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: ProDots Proteins
• Gene: INHBA
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 14 kDa, reducing conditions, 24 kDa, non-reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -80 °C under sterile conditions after reconstitution.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: For a stock solution, reconstitute at 100 μg/mL in 4 mM HCl, or simply roll ProDot^® directly into cell culture medium for immediate use.

Notes

726214.5

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ProDots Recombinant Human/Mouse/Rat Activin A Protein

• Activin A
• activin AB alpha polypeptide
• Activin beta-A chain
• erythroid differentiation factor
• Erythroid differentiation protein
• follicle-stimulating hormone-releasing protein
• FSH-releasing protein
• inhibin beta A chain
• inhibin beta A subunit
• Inhibin, beta-1

Background

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.

Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A - beta A), Activin B (beta B - beta B), Activin AB (beta A - beta B), Inhibin A (alpha - beta A) and Inhibin B (alpha - beta B).

Bioinformatics

• Gene Symbol: INHBA
• Uniprot:
  • P08476()