ProDots Recombinant Human EGF Protein

ProDots® Recombinant Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line.The ED50 for this effect is 20-100 pg/mL.

1 μg/lane of ProDots® Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) conditions and visualized with silver staining, showing a single band at 6 kDa.

Product Discontinued

ProDots Recombinant Human EGF Protein Summary

• Additional Information: Supplied as ready-to-use 5 ug protein balls
• Details of Functionality: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.

  The ED₅₀ for this effect is 20-100 pg/mL.

• Source: E. coli-derived human EGF protein
  Asn971 - Arg1023, with an N-terminal Met
• Accession #: P01133
• N-terminal Sequence: Met
• Protein/Peptide Type: ProDots Proteins
• Gene: EGF
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE:

  6 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -80 °C under sterile conditions after reconstitution.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions:

  For a stock solution, reconstitute at 500 μg/mL in sterile PBS, or simply roll ProDot^® directly into cell culture medium for immediate use.

Notes

726219.1

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ProDots Recombinant Human EGF Protein

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Publications for EGF (PRD236)(2)

Publications using PRD236

• Wong, KG;Cheng, YF;Wu, VH;Kiseleva, AA;Li, J;Poleshko, A;Smith, CL;Epstein, JA; Growth factor-induced activation of MSK2 leads to phosphorylation of H3K9me2S10 and corresponding changes in gene expression Science advances 2024-03-15 [PMID: 38478612] (Bioassay, Human)
• L Ma, Y Tian, T Qian, W Li, C Liu, B Chu, Q Kong, R Cai, P Bai, L Ma, Y Deng, R Tian, C Wu, Y Sun Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression Cell Death & Disease, 2022-05-20;13(5):482. 2022-05-20 [PMID: 35595729] (Bioassay, Human)

Bioinformatics

• Gene Symbol: EGF
• Uniprot:
  • P01133()