Procalcitonin Antibody (4D12.D4.F3.E2.H7.E7) - BSA Free

Western Blot: Procalcitonin Antibody (4D12.D4.F3.E2.H7.E7) [NBP2-44104] - lane 1: MW. Lane 2: Procalcitonin Protein. Load: 5 Ug per lane.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4D12.D4.F3.E2.H7.E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Procalcitonin Antibody (4D12.D4.F3.E2.H7.E7) - BSA Free Summary

• Description: This antibody was purified from concentrated tissue culture supernate by Protein A chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Procalcitonin Antibody (4D12.D4.F3.E2.H7.E7) was produced in mice prepared by repeated immunizations with full-length recombinant human Procalcitonin protein. (Uniprot: P06881)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALCA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:1000-1:2000
• Application Notes: This product was tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Cross-reactivity with Procalcitonin from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Procalcitonin Antibody (4D12.D4.F3.E2.H7.E7) - BSA Free

• CALC1
• CALCA
• Calcitonin 1
• calcitonin gene-related peptide 1
• CGRP
• Katacalcin
• Procalcitonin

Background

Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALCA
• Uniprot:
  • P06881(Human)