Pro-Corticoliberin/Pro-CRH Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Pro-Corticoliberin/Pro-CRH Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Pro‑Corticoliberin/Pro‑CRH
  Leu25-Lys196
  Accession # P06850
• Specificity: Detects human Pro‑Corticoliberin/Pro‑CRH in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pro-Corticoliberin/Pro-CRH Antibody [Alexa Fluor® 350]

• CRF
• CRH
• CRH1
• ProCorticoliberin
• Pro-Corticoliberin

Background

Pro-Corticoliberin (also Pro-CRF and Pro-CRH) is a predicted 19 kDa member of the CRF/Urotensin I family of hormones. It is expressed by placental synctiotrophoblasts, zona fasciculata adrenal cells and paraventricular neurons, and is induced by glucocorticoids. Pro‑Corticoliberin is the precursor to a mature, 41 amino acid (aa) amidated Corticoliberin that induces pituitary ACTH and subsequent cortisol production by the adrenal. Human Pro‑Corticoliberin is 170 aa in length (aa 25‑194). If unprocessed, it undergoes N-terminal amidation to generate a polypeptide 168 aa in length (aa 27‑194). Secreted Pro‑Corticoliberin is both bioactive and binds to 37 kDa Corticoliberin-PB. In the placenta, Pro‑Corticoliberin is only occasionally cleaved to generate mature Corticoliberin (aa 152‑192); in the brain, it undergoes active internal proteolysis to generate multiple fragments, including the 5 kDa mature peptide. Over aa 25‑196, human Pro‑Corticoliberin shares 78% aa identity with mouse Pro‑Corticoliberin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.