PRMT3 Antibody - BSA Free

Western Blot: PRMT3 Antibody [NBP3-35304] - Western blot analysis of lysates from mouse brain, using [KO Validated] PRMT3 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Western Blot: PRMT3 Antibody [NBP3-35304] - Western Blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRMT3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PRMT3 Antibody - BSA Free (NBP3-35304) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1).
  
  Sequence:
  MCSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPCLFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRMT3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:1000 - 1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for PRMT3 Antibody - BSA Free

• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.319
• heterogeneous nuclear ribonucleoprotein methyltransferase-like 3
• Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
• HMT1 hnRNP methyltransferase-like 3 (S. cerevisiae)
• HMT1 hnRNP methyltransferase-like 3
• HRMT1L3
• PRMT3
• protein arginine methyltransferase 3
• protein arginine N-methyltransferase 3

Background

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRMT3