PRL/PTP4A Antibody (334407) [Unconjugated] - Pan Specific

• Reactivity: Hu
• Applications: IHC
• Clone: 334407
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PRL/PTP4A Antibody (334407) [Unconjugated] - Pan Specific Summary

• Immunogen: E. coli-derived recombinant human PRL-3
  Ala2-Met173
  Accession # O75365
• Specificity: Detects human PRL-2 and human PRL-3 with equal affinities in direct ELISAs. In direct ELISAs, this antibody shows 10% cross-reactivity with recombinant human PRL-1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRL/PTP4A Antibody (334407) [Unconjugated] - Pan Specific

• HH72;PRL1;PRL-1;Protein tyrosine phosphatase type IVA 1;PTP(CAAX1);PTP4A1;PTPCAAX1
• PRL/PTP4A

Background

Phosphatase of Regenerating Liver (PRL), also known as PTP4A, dephosphorylates tyrosine residues on proteins. Three PRL variants, PRL-1 (PTP4A1), PRL-2 (PTP4A2), and PRL-3 (PTP4A3) have been cloned and are 75% to 86% homologous. Prenylation at the C-terminal end targets PRL family members to membrane compartments in the cell, especially to early endosomes and the plasma membrane. PRL levels may correlate with cell migration rates and tumor invasiveness, and PRL overexpression is known to cause an increase in the metastatic capability of cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRL/PTP4A Antibody (MAB32191)(4)

Publications using MAB32191

• QY Ding, Y Zhang, L Ma, YG Chen, JH Wu, HF Zhang, X Wang Inhibiting MAPK14 showed anti-prolactinoma effect BMC Endocr Disord, 2020-09-07;20(1):138. 2020-09-07 [PMID: 32894113] (IHC, Human)
• T Tanaka, T Kaida, K Yokoi, S Ishii, N Nishizawa, H Kawamata, H Katoh, T Sato, T Nakamura, M Watanabe, K Yamashita Critical relevance of genomic gains of PRL-3/EGFR/c-myc pathway genes in liver metastasis of colorectal cancer Oncol Lett, 2018-11-19;17(1):1257-1266. 2018-11-19 [PMID: 30655893] (IHC-P, Human)
• Nomogram Incorporating CD44v6 and Clinicopathological Factors to Predict Lymph Node Metastasis for Early Gastric Cancer PLoS ONE, 2016-08-02;11(8):e0159424. 2016-08-02 [PMID: 27482895] (IHC-P, Human)
• Wang Y, Lazo JS Metastasis-associated phosphatase PRL-2 regulates tumor cell migration and invasion. Oncogene, 2011-07-18;31(7):818-27. 2011-07-18 [PMID: 21765462] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • O75365()