PRKAG2 Antibody (3C4) - Azide and BSA Free

Western Blot: PRKAG2 Antibody (3C4) [H00051422-M01] - Analysis of PRKAG2 expression in transfected 293T cell line by PRKAG2 monoclonal antibody (M01), clone 3C4.Lane 1: PRKAG2 transfected lysate (Predicted MW: 37.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PRKAG2 Antibody (3C4) [H00051422-M01] - Analysis of monoclonal antibody to PRKAG2 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: PRKAG2 Antibody (3C4) [H00051422-M01] - Detection limit for recombinant GST tagged PRKAG2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PRKAG2 Antibody (3C4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PRKAG2 Antibody (3C4) - Azide and BSA Free (H00051422-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PRKAG2 (AAH20540, 191 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAE
• Specificity: PRKAG2 - protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKAG2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactive against recombinant protein on ELISA. GST alone used as a negative control. It has also been used for immunofluorescence.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PRKAG2 Antibody (3C4) - Azide and BSA Free

• AAKG
• AAKG2
• AMPK gamma2
• CMH6AMPK subunit gamma-2
• H91620p
• H91620pAMPK gamma2
• PRKAG2
• protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• WPWS5'-AMP-activated protein kinase subunit gamma-2

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAG2
• Entrez:
  • 51422(Human)
• OMIM:
  • 194(Human)
• Uniprot:
  • AAH20540(Human)