Presenilin-2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Presenilin-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Presenilin-2
  Leu2-Thr80
  Accession # P49810
• Specificity: Detects human Presenilin‑2 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Presenilin‑1 N-Terminal Fragment (aa 1‑80) and recombinant human Presenilin‑2 C-Terminal Fragment (aa 308‑388) is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSEN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Presenilin-2 Antibody [Unconjugated]

• AD3LP
• AD4AD3L
• AD5
• E5-1
• EC 3.4.23
• EC 3.4.23.-
• presenilin 2 (Alzheimer disease 4)
• Presenilin2
• Presenilin-2
• PS-2
• PS2CMD1V
• PSEN2
• PSNL2
• STM2STM-2

Background

Presenilin‑2 (PS-2 and PSEN2) is a catalytic subunit of the gamma-secretase complex. Presenilin‑2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Heterogeneous proteolytic processing of Presenilin‑2 generates N-terminal (aa 1-297) and C-terminal (aa 298-248) fragments. Presenilin 1 and Presenilin 2 determine gamma-secretase activity, which is responsible for cleaving the amyloid precursor protein (APP). In turn, APP cleavage can produce amyloid-beta, which is the primary component of plaques in the brains of Alzheimer’s disease patients. Full length, approximately 50 kDa, and the approximately 30 kDa N-terminal fragment of Presenilin-2 were identified by western blotting in extracted rodent pancreas but only the 30 kDa fragment was detected in mouse islets and human insulinoma. Within a sequence correspondent to the immunogen, human PS-2 shares 87% aa sequence identity with mouse and rat PS-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSEN2
• Uniprot:
  • P49810()