Presenilin-2 Antibody [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 669

Presenilin-2 Antibody [Janelia Fluor® 669] Summary

• Immunogen: E. coli-derived recombinant human Presenilin-2
  Leu2-Thr80
  Accession # P49810
• Specificity: Detects human Presenilin‑2 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Presenilin‑1 N-Terminal Fragment (aa 1‑80) and recombinant human Presenilin‑2 C-Terminal Fragment (aa 308‑388) is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSEN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Presenilin-2 Antibody [Janelia Fluor® 669]

• AD3LP
• AD4AD3L
• AD5
• E5-1
• EC 3.4.23
• EC 3.4.23.-
• presenilin 2 (Alzheimer disease 4)
• Presenilin2
• Presenilin-2
• PS-2
• PS2CMD1V
• PSEN2
• PSNL2
• STM2STM-2

Background

Presenilin (PS) facilitates gamma-secretase cleavage of the beta-amyloid precursor protein and the intramembraneous cleavage of Notch1 (1). Presenilin (PS) is essential for the gamma-cleavage required for the generation of the C terminus of amyloid beta-protein (Abeta). The discovery that a deficiency of presenilin 1 (PS1) decreases the production of amyloid beta-protein (Abeta) identified the presenilins as important mediators of the gamma-secretase cleavage of beta-amyloid precursor protein (APP). It has been shown that two conserved transmembrane (TM) aspartates in PS1 are critical for Abeta production, providing evidence that PS either functions as a required diaspartyl cofactor for gamma-secretase or is itself gamma-secretase. Presenilin 2 (PS2) shares substantial sequence and possibly functional homology with PS1. Here, we show that the two TM aspartates in PS2 are also critical for gamma-secretase activity, providing further evidence that PS2 is functionally homologous to PS1 (2). PS are found primarily within intracellular membranes, including the endoplasmic reticulum (ER) and the trans-Golgi network as well as the plasma membrane. PS are also expressed in most cell types throughout development (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSEN2