preproANP Antibody

Western Blot: preproANP Antibody [NBP2-14872] - Expression of cardiac hypertrophic biomarkers in cultured neonatal cardiomyocytes. Secretory preproANP was detected via immunoblot analyses of culture media collected at indicated time points and normalized to total protein measured by Coomassie staining (n = 4 mice). Pictured are representative scans of the preproANP immunoblot and corresponding Coomassie staining. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32377307/) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14872] - Rat heart. ANP stained by preproANP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: preproANP Antibody [NBP2-14872] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody diluted at 1:2000.

Western Blot: preproANP Antibody [NBP2-14872] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with preproANP antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14872] - Human ovarian carcinoma. preproANP antibody dilution: 1:250. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14872] - Mouse heart. ANP stained by preproANP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Am, Sq
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

preproANP Antibody Summary

• Immunogen: Full length human preproANP Recombinant protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Frog (100%), Canine (83%), Porcine (87%), Rabbit (81%), Bovine (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for preproANP Antibody

• ANF
• ANP
• ATFB6
• atrial natriuretic factor prohormone
• atrial natriuretic peptide prohormone
• ATRST2
• CDD
• CDD-ANF
• CDP
• natriuretic peptides A
• PND

Background

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for preproANP Antibody (NBP2-14872)(1)

Publication using NBP2-14872

• Haseli S, Deubel S et al. Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging. Oxid Med Cell Longev 2020-08-05 [PMID: 32377307] (WB, Mouse)

Bioinformatics

• Gene Symbol: NPPA