PRELP Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC215731 and based on accession number NM_002725. The protein contains a C-terminal DDK tag.
Homo sapiens proline/arginine-rich end leucine-rich repeat protein (PRELP), transcript variant 1, mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Lysate Details for PRELP
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for PRELP Overexpression Lysate (Native)
- 55 kDa leucine-rich repeat protein of articular cartilage
- prolargin proteoglycan
- proline arginine-rich end leucine-rich repeat protein
- proline/arginine-rich end leucine-rich repeat protein
- Proline-arginine-rich end leucine-rich repeat protein
The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the same protein as variant 2.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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Additional PRELP Overexpression Lysate (Native) Products
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, CARTILAGE MATRIX PROTEIN
, PROLINE ARGININE-RICH END LEUCINE-RICH REPEAT
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