PRAME Antibody (PRAME/8558R)

Formalin-fixed, paraffin-embedded human melanoma stained with PRAME antibody (PRAME/8558R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: PRAME/8558R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PRAME Antibody (PRAME/8558R) Summary

• Description: Positive Controls: Human testis or melanoma.
  
  Antibody with azide - store at 2 to 8C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa100-509) of human PRAME protein (exact sequence is proprietary)
• Localization: Nucleus. Chromosome. Cytoplasm. Golgi apparatus. Cell membrane
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRAME
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for PRAME Antibody (PRAME/8558R)

• cancer/testis antigen 130
• MAPEOIP4CT130
• melanoma antigen preferentially expressed in tumors
• OIP-4
• Opa-interacting protein 4
• Opa-interacting protein OIP4
• preferentially expressed antigen in melanoma
• Preferentially expressed antigen of melanoma

Background

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRAME