PRAME Antibody (CL5146) - BSA Free

Western Blot: PRAME Antibody (CL5146) [NBP2-61148] - Analysis in human cell line U-2 OS.

Immunocytochemistry/Immunofluorescence: PRAME Antibody (CL5146) [NBP2-61148] - Staining of U-2 OS cells using the Anti-PRAME monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: CL5146
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

PRAME Antibody (CL5146) - BSA Free Summary

• Description: Novus Biologicals Mouse PRAME Antibody (CL5146) - BSA Free (NBP2-61148) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P78395, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRAME
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: ICC/IF ixation/Permeabilization: PFA/Triton X-100
• Control Peptide:

  PRAME Recombinant Protein Antigen (NBP2-61148PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for PRAME Antibody (CL5146) - BSA Free

• cancer/testis antigen 130
• MAPEOIP4CT130
• melanoma antigen preferentially expressed in tumors
• OIP-4
• Opa-interacting protein 4
• Opa-interacting protein OIP4
• preferentially expressed antigen in melanoma
• Preferentially expressed antigen of melanoma

Background

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRAME