PPP4R2 Antibody

Western Blot: PPP4R2 Antibody [NB100-2877] - Detection of Human and Mouse PPP4R2 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody NB100-2877 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunohistochemistry-Paraffin: PPP4R2 Antibody [NB100-2877] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PPP4R2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunoprecipitation: PPP4R2 Antibody [NB100-2877] - Detection of human PPP4R2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody NB100-2877 (lotNB100-2877-2) used for IP at 6 ug per reaction. PPP4R2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2877-1). For blotting immunoprecipitated PPP4R2 ,NB100-2877 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PPP4R2 Antibody Summary

• Description: Novus Biologicals Rabbit PPP4R2 Antibody (NB100-2877) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-PPP4R2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 370 and the C-terminus (residue 417) of human Protein Phosphatase 4, Regulatory Subunit 2 using the numbering given in entry NP_777567.1 (GeneID 151987).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP4R2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100- 1:500
  • Immunohistochemistry-Paraffin 1:100- 1:500
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP4R2 Antibody

• MGC131930
• PP4R2
• protein phosphatase 4, regulatory subunit 2
• serine/threonine-protein phosphatase 4 regulatory subunit 2

Background

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C atcentrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporallocalization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replicationand required for DNA DSB repair

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP4R2 Antibody (NB100-2877)(2)

Publications using NB100-2877

• Bosio Y, Berto G, Camera P et al. PPP4R2 regulates neuronal cell differentiation and survival, functionally cooperating with SMN. Eur J Cell Biol 2012-08-01 [PMID: 22559936]
• Goudreault M, D'Ambrosio LM, Kean MJ et al. A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein. Mol Cell Proteomics 2009-01-01 [PMID: 18782753]

Bioinformatics

• Gene Symbol: PPP4R2
• Entrez:
  • 151987(Human)
  • 232314(Mouse)
• Uniprot:
  • NP_777567.1(Human)