PPP2R5E Knockout HeLa Cell Lysate

Product Discontinued

PPP2R5E Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-1 bp deletion in exon2 and Insertion of the selection cassette in exon2
• Gene: PPP2R5E

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for PPP2R5E Knockout HeLa Cell Lysate

• epsilon isoform of regulatory subunit B56, protein phosphatase 2A
• PP2A B subunit isoform B56-epsilon
• PP2A B subunit isoform B'-epsilon
• PP2A B subunit isoform PR61-epsilon
• PP2A B subunit isoform R5-epsilon
• PP2A, B subunit, B' epsilon
• PP2A, B subunit, B56 epsilon
• PP2A, B subunit, PR61 epsilon
• PP2A, B subunit, R5 epsilon
• PPP2R5E
• protein phosphatase 2, regulatory subunit B (B56), epsilon isoform
• protein phosphatase 2, regulatory subunit B', epsilon isoform
• serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon
• serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilonisoform

Background

The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PPP2R5E