PPP2R5C Antibody (TQ11-1G6)

Product Discontinued

PPP2R5C Antibody (TQ11-1G6) Summary

• Immunogen: Human PPP2R5C [UniProt# Q13362]
• Epitope: C-terminus
• Specificity: This is specific for PP2A (B56 gamma 3 subunit) protein. This does not recognize the B56 gamma 1 isoform of PP2A.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP2R5C
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: This PPP2R5C (TQ11-1G6) antibody is useful for Immunoprecipitation and Western blot. By Western blot, this antibody recognizes overexpressed PPP2R5C protein in transfected cells. Bands at ~50 and 35 kDa may also be seen. Western Blot analysis of endogenous samples is not recommended due to high background. Has been shown to work effectively for Immunoprecipitation, see Lin et al.
• Control:

  293 Whole Cell Lysate
  PP2A alpha Overexpression Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for PPP2R5C Antibody (TQ11-1G6)

• B' alpha regulatory subunit
• B56G
• KIAA0044
• MGC23064
• PP2A B subunit isoform B56-gamma
• PP2A B subunit isoform B'-gamma
• PP2A B subunit isoform PR61-gamma
• PP2A B subunit isoform R5-gamma
• PR61G
• protein phosphatase 2, regulatory subunit B (B56), gamma isoform
• protein phosphatase 2, regulatory subunit B', gamma isoform
• protein phosphatase 2, regulatory subunit B', gamma
• Renal carcinoma antigen NY-REN-29
• serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

Background

Phosphatases are involved in the regulation of MAP kinases. Protein phosphatase 2A (PP2A) is a critical component of the pathway responsible for p38 MAPK up-regulation of SERT catalytic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R5C Antibody (NB100-501)(2)

Publications using NB100-501

• Kuo YC, Huang KY, Yang CH et al. Regulation of phosphorylation of Thr-308 of Akt, cell proliferation, and survival by the B55alpha regulatory subunit targeting of the protein phosphatase 2A holoenzyme to Akt. J Biol Chem;283(4):1882-92. 2008-01-25 [PMID: 18042541] (WB, Mouse)
• Lin SS, Bassik MC, Suh H et al. PP2A regulates BCL-2 phosphorylation and proteasome-mediated degradation at the endoplasmic reticulum. J Biol Chem;281(32):23003-12. 2006-08-11 [PMID: 16717086] (IP, Mouse)

Bioinformatics

• Gene Symbol: PPP2R5C