PPM1E Antibody (SR2281) - BSA Free

Western Blot: PPM1E Antibody (SR2281) [NBP3-22493] - Western blot analysis using NBP3-22493 in SH-SY5Y cell lysate.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: SR2281
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PPM1E Antibody (SR2281) - BSA Free Summary

• Description: Novus Biologicals Rabbit PPM1E Antibody (SR2281) - BSA Free (NBP3-22493) is a recombinant monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human PPM1E (Uniprot #: Q8WY54)
• Specificity: Detects endogenous levels of total PPM1E
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PPM1E
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PPM1E Antibody (SR2281) - BSA Free

• ca(2+)/calmodulin-dependent protein kinase phosphatase N
• CAMKN
• CaMKP-N
• caMKP-nucleus
• DKFZp781F1422
• KIAA1072
• nuclear calmodulin-dependent protein kinase phosphatase
• partner of PIX 1
• partner of PIXA
• partner of PIX-alpha
• POPX1
• PP2CH
• protein phosphatase 1E (PP2C domain containing)
• protein phosphatase 1E
• protein phosphatase, Mg2+/Mn2+ dependent, 1E

Background

The protein encoded by the PPM1E gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase was identified as an interacting protein of Rho guanine nucleotide exchange factors (PIX). PIX proteins are regulators of p21/Cdc42/Rac1-activated kinase 1 (PAK1), a protein kinase mediating biological effects downstream of Rho GTPases. This phosphatase has been shown to block the effects of PAK, and thus inhibit actin stress fiber breakdown and morphological changes driven by cell division cycle 42 (CDC42). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1E