PPM1D Antibody [Unconjugated]

PPM1D was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human PPM1D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10120) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PPM1D was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human PPM1D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10120) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

PPM1D Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PPM1D
  Ser400-Cys605
  Accession # O15297
• Specificity: Detects human PPM1D in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PPM1D Antibody [Unconjugated]

• EC 3.1.3.16
• p53-induced protein phosphatase 1
• PP2C-delta
• PPM1D
• protein phosphatase 1D magnesium-dependent, delta isoform
• protein phosphatase 1D
• protein phosphatase 2C delta isoform
• Protein phosphatase 2C isoform delta
• Protein phosphatase magnesium-dependent 1 delta
• protein phosphatase, Mg2+/Mn2+ dependent, 1D
• wild-type p53-induced phosphatase 1
• Wip1
• WIP1protein phosphatase Wip1

Background

Protein phosphatase 1D, or Wild-type p53-induced phosphatase 1 (WIP-1), is an enzyme encoded by de PPM1D gene. WIP-1 is involved in the negative regulation of p53 expression. WIP1 Phosphatase has been shown to be overexpressed or amplified in human cancers including breast and ovarian cancers, and has been described as a potential therapeutic target in Neuroblastoma; GSK 2830371 is a potent and selective allosteric inhibitor of Wip1 phosphatase (Tocris Cat. 5140).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O15297()