PP2C epsilon/PPM1L Antibody (700612)

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Nb2-11 rat lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PP2C e/PPM1L Monoclonal Antibody (Catalog # MAB6914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C e/PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 700612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PP2C epsilon/PPM1L Antibody (700612) Summary

• Immunogen: E. coli-derived recombinant human PP2C epsilon /PPPM1L
  Asp43-Arg178
  Accession # Q5SGD2
• Specificity: Detects human PP2C epsilon /PPPM1L in direct ELISAS and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PP2C is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1L
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2C epsilon/PPM1L Antibody (700612)

• EC 3.1.3
• EC 3.1.3.16
• MGC132545
• MGC132547
• PP2C epsilon
• PP2CE
• PP2CEProtein phosphatase 2C isoform epsilon
• PP2C-epsilon
• PPM1L
• PPM1-LIKE
• protein phosphatase 1 (formerly 2C)-like
• protein phosphatase 1L
• Protein phosphatase 1-like
• Protein phosphatase 2C epsilon isoform
• protein phosphatase, Mg2+/Mn2+ dependent, 1L

Background

PP2C epsilon, also known as PPM1L, is a 34 kDa (predicted) widely expressed Ser/Thr phosphatase. It consists of a 25 amino acid (aa) extracellular domain, a 17 aa transmembrane segment, and a 318 aa cytoplasmic region (aa 43-360) which contains the phosphatase domain (aa 91-344). Alternate splicing generates additional isoforms of human PP2C epsilon with 179 aa or 127 aa N-terminal truncations or a 166 aa C-terminal truncation. The PP2C epsilon -mediated dephosphorylation of TAK1 and ASK1 is regulated by oxidative stress and inflammatory cytokines. Loss of PP2C epsilon function is associated with the development of metabolic syndrome. Within aa 43-178, human PP2C epsilon shares 99% aa sequence identity with mouse and rat PP2C epsilon .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP2C epsilon/PPM1L Antibody (MAB6914)(1)

Publication using MAB6914

• Alyssa Grogan, Andrew Coleman, Humberto Joca, Henk Granzier, Mark W. Russel, Christopher W. Ward et al. Deletion of obscurin immunoglobulin domains Ig58/59 leads to age-dependent cardiac remodeling and arrhythmia Basic Research in Cardiology 2020-12-01 [PMID: 32910221]

Bioinformatics

• Gene Symbol: PPM1L
• Uniprot:
  • Q5SGD2()