CaM Kinase II delta Antibody (1A8)

Images

 
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody (M02), clone 1A8.Lane 1: CAMK2D transfected lysate(54.1 ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of monoclonal antibody to CAMK2D on NIH/3T3 cell. Antibody concentration 10 ug/ml.
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - CAMK2D monoclonal antibody (M02), clone 1A8 Western Blot analysis of CAMK2D expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D over-expressed 293 cell line, cotransfected with CAMK2D Validated Chimera RNAi ( Cat # H00000817-R01V ) (Lane 2) or non-transfected ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CaM Kinase II delta Antibody (1A8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CAMK2D (AAH32784, 301 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPI
Specificity
CAMK2D (1A8)
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
CAMK2D
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:100-1:2000
Application Notes
Antibody reactivity against recombinant protein and cell lysate for WB. It has also been used for IF, RNAi Validation and ELISA.
Control
NIH 3T3 Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
H00000817-M02 in the following applications:

Publications
Read Publications using
H00000817-M02 in the following applications:

  • WB
    3 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Rat reported in scientific literature (PMID: 22766271).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CaM Kinase II delta Antibody (1A8)

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
  • calcium/calmodulin-dependent protein kinase II delta
  • calcium/calmodulin-dependent protein kinase type II delta chain
  • calcium/calmodulin-dependent protein kinase type II subunit delta
  • CaM kinase II delta subunit
  • CaM Kinase II delta
  • CaM kinase II subunit delta
  • CAMK2D
  • CAMKD
  • CaMK-II delta subunit
  • CaMK-II subunit delta
  • CaM-kinase II delta chain
  • DKFZp686G23119
  • DKFZp686I2288
  • EC 2.7.11
  • EC 2.7.11.17
  • MGC44911

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Four alternatively spliced transcript variants that encode three different isoforms have been characterized to date. Distinct isoforms of this chain have different expression patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaM Kinase II delta Antibody (H00000817-M02)(3)

Reviews for CaM Kinase II delta Antibody (H00000817-M02) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using H00000817-M02:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CaM Kinase II delta H00000817-M02
Enlarge
4
reviewed by:
Verified Customer
WB Rat 08/07/2019
View

Summary

ApplicationWestern Blot
Sample TestedHeart lysates
SpeciesRat
LotH7201-1A8

Comments

CommentsAdult rat heart lysates, reduced and denatured, resolved on 12% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Block with Rockland blocking solution. incubate membrane overnight at 4C with 1:100 dilution of CaMKII-delta antibody. wash with PBS-0.1% tween 20. incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-mouse IgG (H+L). Images taken with Odyssey V3.0.
Western Blot CaM Kinase II delta H00000817-M02
Enlarge
4
reviewed by:
Anne Frei
WB Human 02/11/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CaM Kinase II delta Antibody (H00000817-M02) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Verified Customer
08/07/2019
Application: WB
Species: Rat

Anne Frei
02/11/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CAMK2D
Entrez
OMIM
Uniprot