CaM Kinase II delta Antibody (1A8)

Images

 
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D over-expressed 293 cell line, cotransfected with CAMK2D Validated Chimera RNAi ( Cat # H00000817-R01V ) (Lane 2) or non-transfected ...read more
Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of monoclonal antibody to CAMK2D on NIH/3T3 cell. Antibody concentration 10 ug/ml.
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - CAMK2D monoclonal antibody (M02), clone 1A8 Western Blot analysis of CAMK2D expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody (M02), clone 1A8.Lane 1: CAMK2D transfected lysate(54.1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, RNAi
Clone
1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CaM Kinase II delta Antibody (1A8) Summary

Immunogen
CAMK2D (AAH32784 301 a.a. - 410 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPI
Specificity
CAMK2D (1A8)
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
CAMK2D
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • RNA Inhibition
Application Notes
Antibody reactivity against recombinant protein and cell lysate for WB. It has also been used for IF, RNAi Validation and ELISA.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Reviewed Applications
Read 1 Review rated 4
using
H00000817-M02 in the following applications:

Publications
Read Publication using
H00000817-M02 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CaM Kinase II delta Antibody (1A8)

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
  • calcium/calmodulin-dependent protein kinase II delta
  • calcium/calmodulin-dependent protein kinase type II delta chain
  • calcium/calmodulin-dependent protein kinase type II subunit delta
  • CaM kinase II delta subunit
  • CaM Kinase II delta
  • CaM kinase II subunit delta
  • CAMK2D
  • CAMKD
  • CaMK-II delta subunit
  • CaMK-II subunit delta
  • CaM-kinase II delta chain
  • DKFZp686G23119
  • DKFZp686I2288
  • EC 2.7.11
  • EC 2.7.11.17
  • MGC44911

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Four alternatively spliced transcript variants that encode three different isoforms have been characterized to date. Distinct isoforms of this chain have different expression patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CaM Kinase II delta Antibody (H00000817-M02)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Review for CaM Kinase II delta Antibody (H00000817-M02) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00000817-M02:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot CaM Kinase II delta H00000817-M02
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reviewed by:
Anne Frei
WB Human 02/11/2015
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman

Blocking

Blocking Detailsblocked with 5% milk x 30 min at room temp

Primary Anitbody

Dilution Ratio1:1000 for overnight at 4 deg C in 5% milk

Secondary Antibody

Secondary DescriptionGoat anti rabbit IgG HRP
Secondary Manufacturer Cat#GE healthcare NA934V
Secondary Concentration1:10000

Details

Detection NotesECL for 1 min, exposed film for 1 min-20 min. Positive and negative controls worked. Film is 2 min exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CaM Kinase II delta Antibody (H00000817-M02) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
0
4
1
3
0
2
0
1
0

Anne Frei
02/11/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CAMK2D
Entrez
OMIM
Uniprot