Neurobeachin Antibody - BSA Free

Immunohistochemistry-Paraffin: Neurobeachin Antibody [NBP1-90004] - Analysis in human cerebral cortex and liver tissues using NBP1-90004 antibody. Corresponding NBEA RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Neurobeachin Antibody [NBP1-90004] - Staining of human cerebral cortex, liver, pancreas and stomach using Anti-NBEA antibody NBP1-90004 (A) shows similar protein distribution across tissues to independent antibody NBP1-90003 (B).

Immunocytochemistry/Immunofluorescence: Neurobeachin Antibody [NBP1-90004] - Staining of human cell line SH-SY5Y shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Neurobeachin Antibody [NBP1-90004] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Neurobeachin Antibody [NBP1-90004] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Neurobeachin Antibody [NBP1-90004] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.

Immunohistochemistry-Paraffin: Neurobeachin Antibody [NBP1-90004] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Neurobeachin Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ETTRTGSQPGRNIRQEINSPTSTVVVIPSIPHPSLNHGFLAKLIPEQSFGHSFYKETPAAFPDTIKEKETPTPGEDIQVESSIPHTDSGIGEEQVASILNGAELETSTGPDAMSEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NBEA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
• Control Peptide:

  Neurobeachin Protein (NBP1-90004PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Neurobeachin Antibody - BSA Free

• BCL8Blysosomal trafficking regulator 2
• EC 1.14.14.5
• EC 6.1.1.11
• FLJ10197
• KIAA1544
• Lysosomal-trafficking regulator 2
• LYST2
• neurobeachin
• Protein BCL8B

Background

Neurobeachin binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. It may anchor the kinase to cytoskeletal and/or organelle-associated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NBEA