PP2C epsilon/PPM1L Antibody (700612) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 700612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

PP2C epsilon/PPM1L Antibody (700612) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human PP2C ε/PPPM1L
  Asp43-Arg178
  Accession # Q5SGD2
• Specificity: Detects human PP2C epsilon /PPPM1L in direct ELISAS and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PP2C is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2C epsilon/PPM1L Antibody (700612) [Alexa Fluor® 350]

• EC 3.1.3
• EC 3.1.3.16
• MGC132545
• MGC132547
• PP2C epsilon
• PP2CE
• PP2CEProtein phosphatase 2C isoform epsilon
• PP2C-epsilon
• PPM1L
• PPM1-LIKE
• protein phosphatase 1 (formerly 2C)-like
• protein phosphatase 1L
• Protein phosphatase 1-like
• Protein phosphatase 2C epsilon isoform
• protein phosphatase, Mg2+/Mn2+ dependent, 1L

Background

PP2C epsilon, also known as PPM1L, is a 34 kDa (predicted) widely expressed Ser/Thr phosphatase. It consists of a 25 amino acid (aa) extracellular domain, a 17 aa transmembrane segment, and a 318 aa cytoplasmic region (aa 43-360) which contains the phosphatase domain (aa 91-344). Alternate splicing generates additional isoforms of human PP2C epsilon with 179 aa or 127 aa N-terminal truncations or a 166 aa C-terminal truncation. The PP2C epsilon -mediated dephosphorylation of TAK1 and ASK1 is regulated by oxidative stress and inflammatory cytokines. Loss of PP2C epsilon function is associated with the development of metabolic syndrome. Within aa 43-178, human PP2C epsilon shares 99% aa sequence identity with mouse and rat PP2C epsilon .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.