Testican 3/SPOCK3 Antibody

Western Blot: Testican 3/SPOCK3 Antibody [NBP2-20476] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:5000.

Immunohistochemistry-Paraffin: Testican 3/SPOCK3 Antibody [NBP2-20476] - U373 xenograft, using SPOCK3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Testican 3/SPOCK3 Antibody [NBP2-20476] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:5000.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Testican 3/SPOCK3 Antibody Summary

• Description: Novus Biologicals Rabbit Testican 3/SPOCK3 Antibody (NBP2-20476) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Testican 3/SPOCK3. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPOCK3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Testican 3/SPOCK3 Antibody

• CWCV, and Kazal-like domains proteoglycan 3
• HSAJ1454
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3
• SPOCK3
• TES-3
• Testican 3
• TICN3

Background

Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are components of the extracellular matrix. SPOCK3 encodes a member of a novel Ca(2+)-binding proteoglycan family.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SPOCK3
• Uniprot:
  • Q9BQ16(Human)