POU3F3 Antibody (CL15302) - Azide and BSA Free

Staining in SH-SY5Y cell line with Anti-POU3F3 monoclonal antibody) .

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44291 antibody. Corresponding POU3F3 RNA-seq data are presented for the same tissues.

Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.

Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons and glial cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of mouse cerebral cortex shows strong nuclear positivity in neurons.

Analysis in human cell line HEK 293.

Staining of rat hippocampus shows moderate to strong nuclear positivity in a subset of neurons.

Staining of rat cerebellum shows moderate to strong nuclear positivity in a subset of neurons.

Staining of mouse cerebellum shows strong nuclear positivity in a subset of neurons.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL15302
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

POU3F3 Antibody (CL15302) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse POU3F3 Antibody (CL15302) - Azide and BSA Free (NBP3-43659) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P20264, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POU3F3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for POU3F3 Antibody (CL15302) - Azide and BSA Free

• brain-1
• Brain-specific homeobox/POU domain protein 1
• Brn-1
• BRN1brn-1
• Oct-8
• Octamer-binding protein 8
• Octamer-binding transcription factor 8
• OTF-8
• OTF8oct-8
• POU class 3 homeobox 3
• POU domain class 3, transcription factor 3
• POU domain, class 3, transcription factor 3

Background

POU3F3, also known as POU Class 3 Homeobox 3, is a transcription factor that is primarily expressed in the brain and CNS. POU3F3 interacts with SOX4 and SOX11, and is implicated in the development of neurons. Current research surrounding POU3F3 has shown potential linkages with neuronitis and cerebritis. POU3F3 has also been shown to interact with POU3F4, CAV1, SOX10, and GADD34.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU3F3