Porcine IL-6 Quantikine ELISA Kit

• Reactivity: Po
• Applications: ELISA
• Conjugate: HRP

Porcine IL-6 Quantikine ELISA Kit Summary

• Background: The Quantikine Porcine IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IL-6 and antibodies raised against the recombinant factor. The immunoassay has been shown to accurately quantitate recombinant porcine IL-6. Results obtained using natural porcine IL-...6 show dose response curves that are parallel to the standard curves obtained using recombinant Quantikine Porcine IL-6 kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-6.
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• Specificity: Natural and recombinant porcine IL-6
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL6

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Porcine IL-6 Quantikine ELISA Kit

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, alpha -helical, 22 - 28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 58%, 39% and 39% aa sequence identity with human, mouse, and rat IL-6, respectively (6). Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells (2, 7-29). IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids (2). Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery (30-34). 

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 Ra) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha , triggering IL-6 R alpha association with gp130, and gp130 dimerization (35). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (36). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130- expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 37). 

IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis (2, 5). IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity (1). It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis (1, 2). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of memory B cells and plasma cells (38).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (P6000B)(67)

Publications using P6000B

• Damiano, S;Longobardi, C;Di Napoli, E;Iovane, V;Ferrucci, F;Rizzo, G;Raffaele, A;Rubino, A;Russo, V;Ciarcia, R; Evaluation of Activity of Pro- and Anti-Inflammatory Mediators and Nitrosative Stress in Liver Tissue of Wild Boars (Sus scrofa) Positive for Zearalenone (ZEN) Contamination in Campania Region, Southern Italy Toxins 2025-11-05 [PMID: 41295868] (Porcine)
• Paslawski, R;Kowalczyk, P;Paslawska, U;Wi?niewski, J;Dzi?giel, P;Janiszewski, A;Kiczak, L;Zacharski, M;Gawdzik, B;Kramkowski, K;Szuba, A; Analysis of the Model of Atherosclerosis Formation in Pig Hearts as a Result of Impaired Activity of DNA Repair Enzymes International journal of molecular sciences 2024-02-14 [PMID: 38396961] (Porcine)
• Yang, X;Du, Q;Wang, X;Shi, J;Wang, T;Li, P;Zhong, J;Tong, D;Huang, Y; Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein Veterinary microbiology 2023-08-31 [PMID: 37672899] (Porcine)
• Changsan, N;Sawatdee, S;Suedee, R;Chunhachaichana, C;Srichana, T; Aqueous cannabidiol ?-cyclodextrin complexed polymeric micelle nasal spray to attenuate in vitro and ex vivo SARS-CoV-2-induced cytokine storms International journal of pharmaceutics 2023-05-12 [PMID: 37182795] (Porcine)
• JB Lee, SK Kim, D Han, JW Yoon Mutating both relA and spoT of enteropathogenic Escherichia coli E2348/69 attenuates its virulence and induces interleukin 6 in vivo Frontiers in Microbiology, 2023-03-02;14(0):1121715. 2023-03-02 [PMID: 36937293] (Porcine)
• SY Chang, MH Song, JH Lee, HJ Oh, YJ Kim, JW An, YB Go, DC Song, HA Cho, SY Cho, DJ Kim, MS Kim, HB Kim, JH Cho Phytogenic feed additives alleviate pathogenic Escherichia coli-induced intestinal damage through improving barrier integrity and inhibiting inflammation in weaned pigs Journal of animal science and biotechnology, 2022-09-02;13(1):107. 2022-09-02 [PMID: 36050784] (Porcine)
• R Rissel, M Gosling, J Kamuf, M Renz, R Ruemmler, A Ziebart, EK Hartmann Levosimendan Ameliorates Cardiopulmonary Function but Not Inflammatory Response in a Dual Model of Experimental ARDS Biomedicines, 2022-04-29;10(5):. 2022-04-29 [PMID: 35625767] (Porcine)
• JX Boeckman, S Sprayberry, AM Korn, JS Suchodolsk, C Paulk, K Genovese, RR Rech, PR Giaretta, AK Blick, T Callaway, JJ Gill Effect of chronic and acute enterotoxigenic E. coli challenge on growth performance, intestinal inflammation, microbiome, and metabolome of weaned piglets Scientific Reports, 2022-03-23;12(1):5024. 2022-03-23 [PMID: 35323827] (Porcine)
• M Kasztura, L Kiczak, U Pas?awska, J Bania, A Janiszewsk, A Tomaszek, M Zacharski, A Noszczyk-N, R Pas?awski, A Tabi?, P Kuropka, P Dzi?giel, P Ponikowski Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model International Journal of Molecular Sciences, 2022-01-18;23(3):. 2022-01-18 [PMID: 35162949] (Porcine)
• Y Jiang, W Tong, L Yu, L Li, F Gao, G Li, C Liu, P Chen, Q Shen, Y Zhang, Y Zhou, G Tong Identification of Virulence Associated Region during Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus during Attenuation In Vitro: Complex Question with Different Strain Backgrounds Viruses, 2021-12-27;14(1):. 2021-12-27 [PMID: 35062244] (Porcine)
• VS Michaelsen, RVP Ribeiro, E Brambate, A Ali, A Wang, L Pires, M Kawashima, Y Zhang, A Gazzalle, S Keshavjee, L Del Sorbo, M Cypel A novel pre-clinical strategy to deliver antimicrobial doses of inhaled nitric oxide PLoS ONE, 2021-10-13;16(10):e0258368. 2021-10-13 [PMID: 34644318] (Porcine)
• P Ekanayaka, BH Lee, A Weerawardh, K Chathurang, JH Park, JS Lee Inhibition of MAVS Aggregation-Mediated Type-I Interferon Signaling by Foot-and-Mouth Disease Virus VP3 Viruses, 2021-09-06;13(9):. 2021-09-06 [PMID: 34578357] (Porcine)
• PD Leiphrakpa, HR Weber, A McCain, RR Matas, EM Duarte, KL Buesing A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome Respiratory Research, 2021-07-07;22(1):198. 2021-07-07 [PMID: 34233680] (Porcine)
• M Al-Obeidal, D Jarkovská, L Valešová, J Horák, J Jedli?ka, L Nalos, J Chvojka, J Švíglerová, J Kuncová, J Beneš, M Mat?jovi?, M Štengl SOFA Score, Hemodynamics and Body Temperature Allow Early Discrimination between Porcine Peritonitis-Induced Sepsis and Peritonitis-Induced Septic Shock Journal of personalized medicine, 2021-02-28;11(3):. 2021-02-28 [PMID: 33670874] (Porcine)
• J Bhogal, AL Solevåg, M O'Reilly, TF Lee, C Joynt, LK Hornberger, GM Schmölzer, PY Cheung Hemodynamic effects of high frequency oscillatory ventilation with volume guarantee in a piglet model of respiratory distress syndrome PLoS ONE, 2021-02-16;16(2):e0246996. 2021-02-16 [PMID: 33592023] (Porcine)
• J Kamuf, A Garcia Bar, A Ziebart, K Frauenknec, K Folkert, J Schwab, R Ruemmler, M Renz, D Cana, SC Thal, EK Hartmann Experimental lung injury induces cerebral cytokine mRNA production in pigs PeerJ, 2020-12-09;8(0):e10471. 2020-12-09 [PMID: 33354426] (Porcine)
• S Halvachiza, M Teuben, M Lempert, Y Kalbas, N Cesarovic, M Lipiski, E Benninger, P Cinelli, R Pfeifer, HC Pape Protective effects of new femoral reaming techniques (Reamer irrigator aspirator, RIA I and II) on pulmonary function and posttraumatic contusion (CT morphology) - results from a standardized large animal model Injury, 2020-10-06;0(0):. 2020-10-06 [PMID: 33046248] (Porcine)
• GH Shim, SY Kim, PY Cheung, TF Lee, M O'Reilly, GM Schmölzer Effects of sustained inflation pressure during�neonatal cardiopulmonary resuscitation of asphyxiated piglets PLoS ONE, 2020-06-23;15(6):e0228693. 2020-06-23 [PMID: 32574159] (Porcine)
• S McCleary, R Strong, RR McCarthy, JC Edwards, EL Howes, LM Stevens, PJ Sánchez-Co, A Núñez, S Watson, AJ Mileham, SG Lillico, C Tait-Burka, C Proudfoot, M Ballantyne, CBA Whitelaw, F Steinbach, HR Crooke Substitution of warthog NF-kappaB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus Sci Rep, 2020-06-02;10(1):8951. 2020-06-02 [PMID: 32488046] (Porcine)
• S Spring, H Premathila, U DeSilva, C Shili, S Carter, A Pezeshki Low Protein-High Carbohydrate Diets Alter Energy Balance, Gut Microbiota Composition and Blood Metabolomics Profile in Young Pigs Sci Rep, 2020-02-24;10(1):3318. 2020-02-24 [PMID: 32094453] (Porcine)
• SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model PLoS ONE, 2019-06-27;14(6):e0218624. 2019-06-27 [PMID: 31247004] (Porcine)
• R Saleri, V Cavalli, L Ferrari, G Ogno, E Canelli, P Martelli, P Borghetti Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1) infection Res. Vet. Sci., 2019-04-11;124(0):263-269. 2019-04-11 [PMID: 31003008] (Porcine)
• S Boehme, EK Hartmann, T Tripp, SC Thal, M David, D Abraham, JE Baumgardne, K Markstalle, KU Klein PO2 oscillations induce lung injury and inflammation Crit Care, 2019-03-27;23(1):102. 2019-03-27 [PMID: 30917851] (Porcine)
• M Galasso, JJ Feld, Y Watanabe, M Pipkin, C Summers, A Ali, R Qaqish, M Chen, RVP Ribeiro, K Ramadan, L Pires, VS Bagnato, C Kurachi, V Cherepanov, G Moonen, A Gazzalle, TK Waddell, M Liu, S Keshavjee, BC Wilson, A Humar, M Cypel Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion Nat Commun, 2019-01-29;10(1):481. 2019-01-29 [PMID: 30696822] (Porcine)
• L Zhang, X Wang, S Chen, S Wang, Z Tu, G Zhang, H Zhu, X Li, J Xiong, Y Liu Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways Int J Mol Sci, 2018-11-21;19(11):. 2018-11-21 [PMID: 30469452] (Porcine)
• A Miko?ajczy, D Z?otkowska Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis Int J Mol Sci, 2018-10-22;19(10):. 2018-10-22 [PMID: 30360353] (Porcine)
• C Wille, T Eiseler, ST Langenberg, J Richter, K Mizuno, P Radermache, U Knippschil, M Huber-Lang, T Seufferlei, S Paschke PKD regulates actin polymerization, neutrophil deformability, and transendothelial migration in response to fMLP and trauma J. Leukoc. Biol., 2018-04-14;0(0):. 2018-04-14 [PMID: 29656400] (Porcine)
• M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome BMC Vet. Res., 2018-03-12;14(1):83. 2018-03-12 [PMID: 29530043] (Porcine)
• T Duburcq, A Durand, A Tournoys, V Gnemmi, V Gmyr, F Pattou, M Jourdain, F Tamion, E Besnier, S Préau, E Parmentier, D Mathieu, J Poissy, R Favory Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study Ann Intensive Care, 2018-02-14;8(1):24. 2018-02-14 [PMID: 29445877] (Porcine)
• V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels Influence of zinc supplementation on immune parameters in weaned pigs J Trace Elem Med Biol, 2018-01-31;0(0):. 2018-01-31 [PMID: 29402502] (ELISA Capture, Porcine)
• W Gozdzik, S Zielinski, M Zielinska, K Ratajczak, P Skrzypczak, S Rodziewicz, A Kübler, K Löfström, P Dziegiel, M Olbromski, B Adamik, S Ryniak, P Harbut, J Albert, C Frostell Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177514. 2018-01-01 [PMID: 29376749] (Porcine)
• Characterization of blunt chest trauma in a long-term porcine model of severe multiple trauma Sci Rep, 2016-12-21;6(0):39659. 2016-12-21 [PMID: 28000769] (Porcine)
• Angela J Westover An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome J Diabetes Res, 2016-10-13;2016(0):3486727. 2016-10-13 [PMID: 27819007] (Porcine)
• Rolf Rossaint Septic porcine blood does not further activate coagulation during in vitro membrane oxygenation Eur J Cardiothorac Surg, 2017-03-01;0(0):. 2017-03-01 [PMID: 27806995] (Porcine)
• Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression. J Virol, 2015-01-21;89(7):3723-36. 2015-01-21 [PMID: 25609823] (Porcine)
• Ziebart A, Hartmann E, Thomas R, Liu T, Duenges B, Schad A, Bodenstein M, Thal S, David M Low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs. Respir Res, 2014-09-06;15(0):101. 2014-09-06 [PMID: 25189285] (Porcine)
• Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig. Mol. Ther., 2012-03-27;20(6):1204-11. 2012-03-27 [PMID: 22453765] (Porcine)
• Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs. Crit. Care Med., 2012-01-01;40(1):162-8. 2012-01-01 [PMID: 21926613] (Porcine)
• Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis. Crit. Care Med., 2010-06-01;38(6):1467-74. 2010-06-01 [PMID: 20400898] (Porcine)
• Jeong HJ, Song YJ, Lee SW Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. Clin. Vaccine Immunol., 2010-02-03;17(4):503-12. 2010-02-03 [PMID: 20130128] (Porcine)
• Fernandez-Sainz I, Gladue DP, Holinka LG Mutations in classical swine fever virus NS4B affect virulence in swine. J. Virol., 2009-11-18;84(3):1536-49. 2009-11-18 [PMID: 19923180] (Porcine)
• McILwain RB, Timpa JG, Kurundkar AR Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine. Lab. Invest., 2009-11-09;90(1):128-39. 2009-11-09 [PMID: 19901912] (Porcine)
• Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J., 2009-10-19;24(3):712-22. 2009-10-19 [PMID: 19841036] (Porcine)
• Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine. J. Interferon Cytokine Res., 2009-11-01;0(0):. 2009-11-01 [PMID: 19642909] (Porcine)
• Luna CM, Bruno DA, Garcia-Morato J, Mann KC, Risso Patron J, Sagardia J, Absi R, Garcia Bottino M, Marchetti D, Famiglietti A, Baleztena M, Biancolini C Effect of linezolid compared with glycopeptides in methicillin-resistant Staphylococcus aureus severe pneumonia in piglets. Chest, 2009-01-13;135(6):1564-71. 2009-01-13 [PMID: 19141525] (Porcine)
• Roesner JP, Petzelbauer P, Koch A, Tran N, Iber T, Vagts DA, Scheeren TW, Vollmar B, Noldge-Schomburg GE, Zacharowski K Bbeta15-42 (FX06) reduces pulmonary, myocardial, liver, and small intestine damage in a pig model of hemorrhagic shock and reperfusion. Crit. Care Med., 2009-02-01;37(2):598-605. 2009-02-01 [PMID: 19114899] (Porcine)
• Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood. Infect. Immun., 2008-12-01;77(2):725-32. 2008-12-01 [PMID: 19047409] (Porcine)
• Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery. Acta Vet. Scand., 2008-08-12;50(0):32. 2008-08-12 [PMID: 18700003] (Porcine)
• Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A Effects of glucocorticoids in ventilated piglets with severe pneumonia. Eur. Respir. J., 2008-05-28;32(4):1037-46. 2008-05-28 [PMID: 18508831] (Porcine)
• Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model. J. Immunol., 2008-05-01;180(9):6346-53. 2008-05-01 [PMID: 18424758] (Porcine)
• Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response. Eur. Respir. J., 2007-09-05;30(6):1167-72. 2007-09-05 [PMID: 17804447] (Porcine)
• Luna CM, Baquero S, Gando S, Patron JR, Morato JG, Sibila O, Absi R, Famiglietti A, Vay CA, Von Stecher F, Agusti C, Torres A Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation. Chest, 2007-08-01;132(2):523-31. 2007-08-01 [PMID: 17699131] (Porcine)
• Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury. Crit. Care Med., 2007-07-01;35(7):1730-5. 2007-07-01 [PMID: 17522584] (Porcine)
• Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH Sex difference in link between interleukin-6 and stress. Endocrinology, 2007-05-17;148(8):3758-64. 2007-05-17 [PMID: 17510233] (Porcine)
• Bando T, Morikawa Y, Komori T, Senba E Complete overlap of interleukin-31 receptor A and oncostatin M receptor beta in the adult dorsal root ganglia with distinct developmental expression patterns. Neuroscience, 2006-08-22;142(4):1263-71. 2006-08-22 [PMID: 16926070] (Porcine)
• Moller S, Lauridsen C Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages. Cytokine, 2006-08-17;35(1):6-12. 2006-08-17 [PMID: 16914322] (Porcine)
• Nimjee SM, Keys JR, Pitoc GA, Quick G, Rusconi CP, Sullenger BA A novel antidote-controlled anticoagulant reduces thrombin generation and inflammation and improves cardiac function in cardiopulmonary bypass surgery. Mol. Ther., 2006-06-09;14(3):408-15. 2006-06-09 [PMID: 16765093] (Porcine)
• Jeng MJ, Soong WJ, Lee YS, Chang HL, Shen CM, Wang CH, Yang SS, Hwang B Effects of therapeutic bronchoalveolar lavage and partial liquid ventilation on meconium-aspirated newborn piglets. Crit. Care Med., 2006-04-01;34(4):1099-105. 2006-04-01 [PMID: 16484898] (Porcine)
• Lauritzen B, Lykkesfeldt J, Djurup R, Flodgaard H, Svendsen O Effects of heparin-binding protein (CAP37/azurocidin) in a porcine model of Actinobacillus pleuropneumoniae-induced pneumonia. Pharmacol. Res., 2005-06-01;51(6):509-14. 2005-06-01 [PMID: 15829430] (Porcine)
• Waldow T, Alexiou K, Witt W, Albrecht S, Wagner F, Knaut M, Matschke K Protection against acute porcine lung ischemia/reperfusion injury by systemic preconditioning via hind limb ischemia. Transpl. Int., 2005-02-01;18(2):198-205. 2005-02-01 [PMID: 15691273] (Porcine)
• Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide. J. Nutr., 2005-02-01;135(2):239-44. 2005-02-01 [PMID: 15671220] (Porcine)
• Escobar J, Van Alstine WG, Baker DH Decreased protein accretion in pigs with viral and bacterial pneumonia is associated with increased myostatin expression in muscle. J. Nutr., 2004-11-01;134(11):3047-53. 2004-11-01 [PMID: 15514274] (Porcine)
• Waldow T, Alexiou K, Witt W, Wagner FM, Gulielmos V, Matschke K, Knaut M Attenuation of reperfusion-induced systemic inflammation by preconditioning with nitric oxide in an in situ porcine model of normothermic lung ischemia. Chest, 2004-06-01;125(6):2253-9. 2004-06-01 [PMID: 15189949] (Porcine)
• Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells. J. Gen. Virol., 2004-04-01;85(0):1029-37. 2004-04-01 [PMID: 15039545] (Porcine)
• Wulster-Radcliffe MC, Ajuwon KM, Wang J, Christian JA, Spurlock ME Adiponectin differentially regulates cytokines in porcine macrophages. Biochem. Biophys. Res. Commun., 2004-04-09;316(3):924-9. 2004-04-09 [PMID: 15033490] (Porcine)
• Krishnan RK, Meyers PA, Worwa C, Goertz R, Schauer G, Mammel MC Standardized lung recruitment during high frequency and conventional ventilation: similar pathophysiologic and inflammatory responses in an animal model of respiratory distress syndrome. Intensive Care Med, 2004-03-02;30(6):1195-203. 2004-03-02 [PMID: 14997292] (Porcine)
• Eissner B, Matz K, Smorodchenko A, Roschmann A, v Specht BU Chronic porcine two-hit model with hemorrhagic shock and Pseudomonas aeruginosa sepsis. Eur Surg Res, 2002-01-01;34(1):61-7. 2002-01-01 [PMID: 11867903] (Porcine)

FAQs for IL-6 (P6000B). (Showing 1 - 2 of 2 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL6