PON2 Antibody

Western Blot: PON2 Antibody [NBP1-52005] - HepG2 lysate (35ug protein in RIPA buffer) using NBP1-52005 at 0.2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PON2 Antibody [NBP1-52005] - 3.75ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PON2 Antibody Summary

• Description: Novus Biologicals Goat PON2 Antibody (NBP1-52005) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PON2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKPRARELRIS corresponding to internal region according to NP_000296.2, NP_001018171.1.
• Epitope: C-EKPRARELRIS
• Specificity: This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2; NP_001018171.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PON2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 ug/ml
• Application Notes: Western blot: Approx 40kDa double band observed in lysates of HepG2 (calculated MW of 39.4kDa according to NP_000296.2 and of 38.0kDa according to NP_001018171.1). Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide
  IHC: Paraffin embedded Human Placenta.

Reactivity Notes

Expected from sequence similarity: Rat, Porcine, Canine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PON2 Antibody

• A-esterase 2
• Aromatic esterase 2
• EC 3.1.1.2
• EC 3.1.1.81
• paraoxonase 2
• paraoxonase nirs
• PON 2
• PON2
• Serum Aryldialkylphosphatase 2
• serum paraoxonase/arylesterase 2

Background

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PON2 Antibody (NBP1-52005)(1)

Publication using NBP1-52005

• Shin BS. Paraoxonase gene polymorphism in south-western Korean population. J Korean Med Sci 2009-08-01 [PMID: 19654933]

Bioinformatics

• Gene Symbol: PON2
• Entrez:
  • 5445(Human)
  • 296851(Rat)
• Uniprot:
  • NP_000296.2(Human)