PON1 Antibody

Western Blot: PON1 Antibody [NBP1-26401] - Analysis of PON1 in Human Liver lysate (35ug protein in RIPA buffer) using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PON1 Antibody Summary

• Description: Novus Biologicals Goat PON1 Antibody (NBP1-26401) is a polyclonal antibody validated for use in WB and ELISA. Anti-PON1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EEPKVTQVYAEN corresponding to internal region according to NP_000437.3.
• Epitope: Internal region (EEPKVTQVYAEN)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PON1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human liver lysates (calculated MW of 39.7 kDa band according to NP_000437.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PON1 Antibody

• A-esterase 1
• Aromatic esterase 1
• arylesterase B-type
• EC 3.1.1.2
• EC 3.1.1.81
• EC 3.1.8.1
• ESA
• ESAesterase A
• K-45
• MVCD5
• paraoxonase 1
• PON 1
• PON1
• PONparaoxonase B-type
• serum aryldiakylphosphatase
• Serum aryldialkylphosphatase 1
• serum paraoxonase/arylesterase 1

Background

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PON1 Antibody (NBP1-26401)(1)

Publication using NBP1-26401

• Bhattacharyya T, Nicholls SJ, Topol EJ et al. Relationship of paraoxonase 1 (PON1) gene polymorphisms and functional activity with systemic oxidative stress and cardiovascular risk. JAMA 2008-03-19 [PMID: 18349088]

Bioinformatics

• Gene Symbol: PON1
• Entrez:
  • 5444(Human)
• Uniprot:
  • NP_000437.3(Human)